Professor
University Distinguished Service Professor; Project Leader, Division Johns Hopkins Medicine Singapore
BA, 1951; MD, 1955; Stanford University School of Medicine
School of Medicine
Pharmacology and Molecular Sciences
- • Antigen epitope discovery and characterization, and application to epitope-based vaccines and di-agnostics: Working in collaboration with Dr. Vladimir Brusic, Head, Knowledge Discovery Department, Institute for Infocomm Research, Singapore, we are using immunoinformatic programs for prediction of human and mouse T cell epitopes, and applying these methodologies to the development of epi-tope-based vaccines and diagnostics
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• LAMP molecular adjuvant strategy for antigen targeting to the MHC II compartment of antigen presenting cells: A major discovery of this laboratory has been the design of DNA vaccines as a recombinant gene chimera, containing the antigen sequence attached to the lysosome-associated membrane protein (LAMP) that is co-localized with MHC II of antigen presenting cells.
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•Epitope-based, LAMP chimera DNA anti-virus vaccines: The epitope discovery and LAMP targeting applications have been combined as a platform technology for the development of novel forms of DNA encoded vaccines that will enhance the immune response without additional product formulations. This technology is being applied to vaccines for HIV-1, dengue, influenza, and hTERT.
- Alpha Omega Alpha, Stanford University School of Medicine,1954
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Research Fellow, American Heart Association, l957-59
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Markle Scholar in Medical Sciences, Stanford University School of Medicine, 1960-65
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Career Scientist, Health Research Council of the City of New York, 1962-71
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Siegfried Ullmann Professor of Molecular Biology, Albert Einstein College of Medicine Fellow, John Simon Guggenheim Memorial Foundation, 1971-72
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Recipient of Johnson and Johnson Focused Giving Award, 1982-1987
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Fellow, American Association for the Advancement of Science, 1994
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University Distinguished Service Professor, Johns Hopkins Univ School of Medicine, 2001
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Adjunct Professor of Medicine, National University of Singapore, 2002
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NIAID R37 Merit Award, 2002
Research Projects
Johns Hopkins in Singapore
Johns Hopkins Singapore (JHS) was established by The Johns Hopkins University in 1998 as its base of medical operations in South East Asia. JHS has a tripartite mission of research, education and patient care, integrating basic translational and clinical research components.
Research and...
Large Scale epitope mapping (Immune Epitope Database (IEDB))
Map epitopes of category A to C agents for vaccine development and diagnostic tests.
1. PI Brusic - Large-scale discovery of T-Cell epitopes will be applied to selected NIAID category A-C bioterrorism agents. Current computational methods of Hidden Markov models and artificial neural networks...
- • Khan, AM, Heiny AT, Lee KX, Srinivasan KN, Tan TW, August JT and Brusic V. Large-Scale Sequence Analysis of Dengue Virus: Viral Genetic and Antigenic Diversity. Submitted
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• Zhang, GL,, Petrovsky N, Chee KK, August JT and Brusic V. PREDTAP: a system for prediction of peptide binding to the human transporter associated with antigen processing. Immunome Research, in press
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• Zhang, GL, Srinivasan KN, Khan, AM, Chee KK, August JT and Brusic V. Hotspot Hunter: a computational system for prediction of immunological hotspots. Submitted
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• Tong, JC, Zhang, GL, Tan, TW, August, JT, Brusic, V and Ranganathan, S Prediction of HLA-DQ3.2{beta} ligands: evidence of multiple registers in class II binding peptides. Bioinformatics, in press, 2006
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• Anwar A, August JT, and Too HP (2006) A Stem Loop-Mediated Reverse Transcription-Real-Time PCR For The Selective Detection And Quantification Of The Replicative Strand Of A RNA Virus Analytical Biochemistry, in press
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• Gupta V, Tabiin TM, Sun K, Chandrasekaran A, Anwar A, Yang K, Chikhlikar P, Salmon J, Brusic V, Marques ETA, Srinivasan KN, and August JT, SARS Coronavirus Nucleocapsid Immunodominant T-cell Epitopes are Common to Both Exogenous Recombinant and Endogenous DNA Encoded Immunogens. Virology, 347, 127-139, 2006
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• Eskelinen, E-L., Cuervo, A.M., Taylor, M.R.G., Nishino, I., Blum, J.S., Dice, J. F., Sandoval, I.V., Bonifacino, J., Lippincott-Schwartz, J., August, J.T., and Saftig, P., Unifying nomenclature for the isoforms of the lysosomal membrane protein LAMP-2. Traffic, 6:1058-1061, 2005
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• Zhang GL, Khan AM, Srinivasan KN, August JT and Brusic V. Neural models for predicting viral vaccine targets. Bioinfo Comp Biol, 3:1207-1225, 2005
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• Zhang GL, Srinivasan KN, Veeramani, A, August JT and Brusic V. PREDBALB/c: a system for prediction of peptide binding to the H2d molecules, a haplotype of the BALB/c mouse. Nucleic Acids Res 33, W180-183, 2005
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• Brusic, V., August, J.T., and Petrovsky, N., Information Technologies for Vaccine Research, Expert Rev. Vaccines 4, 407-417, 2005
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• Zhang, G. L., Khan, A. M., Srinivasan, K. N., August, J. T., and Brusic, V. MULTIPRED: a computational system for prediction of promiscuous HLA binding peptides. Nucleic Acids Res 33, W172-179, 2005.
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• Anwar, A., Chandrasekaran, A., Ng, M. L., Marques, E., and August, J. T., West Nile Premembrane-Envelope Genetic Vaccine Encoded as a Chimera Containing the Transmembrane and Cytoplasmic Domains of a Lysosome-Associated Membrane Protein: Increased Cellular Concentration of the Transgene product, Targeting to the MHC II Compartment, and Enhanced Neutralizing Antibody Response, Virology 332:66-77, 2005
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• Srinivasan KN, Brusic V and August JT. New Technologies for Vaccine Development. Drug Dev Res, 62:387-396, 2004
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• Brusic V., August J.T. The changing field of vaccine development in the genomics era. Pharmacogenomics 5, 597-600, 2004
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• Srinivasan KN, Zhang G, Khan AM, August JT and Brusic V. Prediction of Class I T-cell epitopes: Evidence of presence of immunological hot spots inside antigens. Bioinformatics, 20:Suppl. 1, I297-I302, 2004
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• Akasaki, K. Nakamura, N., Tsukui, N., Yokota, S., Murata, S., Katoh, R., Michihara, A., Tsuji, H., Marques, E.T, Jr., and August, J.T., Human dendritic cell lysosome-associated membrane protein expressed in lung type II pneumocytes. Arch. Biochem Biophys, 425:147-157, 2004
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• de Arruda, L.B, Chikhlikar, P.R., August, J. T., and Marques, E.T Jr., DNA vaccine encoding human immunodeficiency virus-1 Gag, targeted to the major histocompatibility complex II compartment by lysosomal-associated membrane protein, elicits enhanced long-term memory response. Immunology 112:126-33, 2004
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• Chikhlikar, P.R., Barros de Arruda, L., Agrawal, S., Byrne, B., Guggino, W., August, J. T., and Marques Jr., E.T.A., Inverted terminal repeat sequences of adeno-associated virus enhance the antibody and CD8+ responses to a HIV-1 p55Gag/LAMP DNA vaccine chimera, Virology, 323: 220-232, 2004
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• Marques, E. T.A.Jr., Chikhlikar, P., Barros de Arruda, L., Leao, I.C., Lu, Y.,Wong, J., Chen, J-S., Byrne, B., and August, J.T. HIV-1 p55Gag encoded in the lysosome-associated membrane protein-1 as a DNA plasmid vaccine chimera is highly expressed, traffics to the major histocompatibility class II compartment, and elicits enhanced immune responses. J. Biol. Chem., 278: 37926-37936, 2003
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